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SUMMARY:Prof. Anthony Poole\, University of Auckland (NZ): Experimental ev
 olution sheds insights on the intracellular lifestyle
DTSTART;TZID=Europe/Berlin:20241119T090000
DTEND;TZID=Europe/Berlin:20241119T100000
DTSTAMP:20260613T215003Z
UID:0d7910b2b5274356b175ed0dfb2448e5@www2.hrz.uni-marburg.de
CREATED:20240913T110822Z
DESCRIPTION:https://www2.hrz.uni-marburg.de/en/synmikro/grk2937/whats-on/d
 ates-and-events/prof-anthony-poole-university-of-auckland-nz\nSpeaker Seri
 es. Abstract: Intracellularity leads to some quite unusual changes to geno
 mes and to nucleotide metabolism. I will present the results of two studie
 s that make use of experimental evolution for to examine the evolution of 
 two phenomena. In the first project\, I will present work from our lab on 
 the impact of knocking out genes for ribonucleotide reduction in the bacte
 rium E. coli. We document the dependence of these bacteria on externally-s
 upplied deoxyribonucleosides\, mimicking the loss of this process in some 
 intracellular bacterial species\, including Ureaplasma\, Borrelia and Buch
 nera. Our experiment revealed subsequent gene losses that were also found 
 to have naturally occurred in these species. The paradox we observed was t
 hat the gene losses that emerged following loss of ribonucleotide reductio
 n prevented the potential for evolution to make use of an alternative mech
 anism for dNTP synthesis\, suggesting that a short term benefit trumps the
  long term potential in this system. The second project involves the seria
 l bottlenecking of E. coli to see whether genetic drift hastens the emerge
 nce of genomic errors that can be corrected via slippage (during) or via e
 diting (after) transcription. Such processes are quite common in intracell
 ular bacteria such as Buchnera\, but also are found in eukaryotic organell
 es of bacterial origin. Our results show that\, under conditions favouring
  genetic drift\, we do indeed observe an increased probability of the evol
 ution of these processes. We conclude that our experiments support the ide
 a of constructive neutral evolution\, a process by which genetic drift lea
 ds to more complex molecular processes with no immediate advantage. In bot
 h cases\, the ancestral state did not require the capacity for slippage or
  editing\, but\, at the end of the experiment\, loss of such processes wou
 ld unmask sublethal mutations - the genomes must now be corrected during g
 ene expression to avoid this.
LAST-MODIFIED:20241018T094638Z
LOCATION:SYNMIKRO Lecture Hall
URL:https://www2.hrz.uni-marburg.de/en/synmikro/grk2937/whats-on/dates-and
 -events/prof-anthony-poole-university-of-auckland-nz
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