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SUMMARY:Prof. Tung Le\, Centre for Microbial Interactions\, Norwich: The s
 ilence of the genes: How a molecular clamp shuts down long-range gene expr
 ession in multi-drug resistance plasmids?
DTSTART;TZID=Europe/Berlin:20250415T090000
DTEND;TZID=Europe/Berlin:20250415T100000
DTSTAMP:20260613T234057Z
UID:73359b7be02c44abbad8c2b6a000bb9f@www2.hrz.uni-marburg.de
CREATED:20241205T125047Z
DESCRIPTION:https://www2.hrz.uni-marburg.de/en/synmikro/grk2937/whats-on/d
 ates-and-events/grk2937_speaker-series_tung-le\nSpeaker Series. Long-range
  gene regulation in bacteria is rare and typically attributed to DNA loopi
 ng and supercoiling. In this study\, we use a combination of biophysical a
 pproaches—including X-ray crystallography and single-molecule analysis
 —to investigate the KorB–KorA system in Escherichia coli. Our findings
  reveal that long-range gene silencing on the plasmid RK2\, a key driver o
 f multi-drug resistance in diverse Gram-negative bacteria\, is facilitated
  cooperatively by the DNA-sliding clamp KorB and the clamp-locking protein
  KorA. We demonstrate that KorB functions as a CTPase clamp capable of ent
 rapping and sliding along DNA to reach target promoters up to 1.5 kb away.
  By resolving the tripartite crystal structure of the KorB–KorA–DNA co
 mplex\, we show that KorA locks KorB into a closed clamp state. When bound
  to DNA\, KorA enhances repression by halting KorB’s sliding at target p
 romoters\, thereby blocking RNA polymerase holoenzyme access. Overall\, ou
 r study elucidates the molecular mechanism underlying KorB’s dual role a
 s a DNA-sliding clamp and a co-repressor\, offering an alternative model f
 or long-range gene regulation in bacteria. In the final part of my talk\, 
 I will discuss the evolutionary adaptation of CTPase enzymes to perform a 
 wide range of biological functions.
LAST-MODIFIED:20250317T104949Z
LOCATION:SYNMIKRO Lecture Hall
URL:https://www2.hrz.uni-marburg.de/en/synmikro/grk2937/whats-on/dates-and
 -events/grk2937_speaker-series_tung-le
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DTSTART:20250330T030000
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