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SUMMARY:Prof. Patrick Pausch\, Vilnius University:  Of DNA making and RNA 
 breaking in bacterial immunity
DTSTART;TZID=Europe/Berlin:20250616T131500
DTEND;TZID=Europe/Berlin:20250616T141500
DTSTAMP:20260614T042928Z
UID:a0100f1f805d47b9935b4eed2fe009ff@www2.hrz.uni-marburg.de
CREATED:20241205T125047Z
DESCRIPTION:https://www2.hrz.uni-marburg.de/en/synmikro/grk2937/whats-on/d
 ates-and-events/grk2937_speaker-series_patrick-pausch\nSpeaker Series. Abs
 tract:  Reverse transcriptases (RTs) synthesize DNA from RNA templates. Pr
 okaryotic RTs are linked to mobile genetic elements (MGE)\, either facilit
 ating or interfering with their propagation. The ongoing conflict between 
 viruses and their host has favoured the evolution of diverse RT-based syst
 ems that provide bacteria with immunity against phages\, such as retrons\,
  abortive phage infection systems (Abi)\, CRISPR-Cas RTs and unknown group
  (UG) systems. In this talk\, I will present our recent efforts in underst
 anding the retron system Eco2 (Ec67). How Eco2 mediates anti-phage defense
  by reverse transcribing non-coding RNA into multicopy single-stranded DNA
  (msDNA) is unknown. Unlike most other retrons\, Eco2 employs a single pro
 tein that couples a reverse transcriptase (RT) and RNase H-like topoisomer
 ase primase (TOPRIM). Cryo-EM reveals the unique homotrimeric nucleoprotei
 n structure of Eco2\, which supports an msDNA-dependent regulation of Eco2
 's RT-TOPRIM fusion protein. Purification of complex formation intermediat
 es identifies the ribosomal protein S1 as a putative ncRNA-chaperone and t
 ranslation regulator. Finally\, we show that Eco2's broad defense against 
 various phages is triggered by a phage-encoded endonuclease that degrades 
 the msDNA. msDNA decay in turn activates the TOPRIM for tRNA cleavage\, in
  turn halting protein production for abortive infection. These findings pr
 ovide insights into the biogenesis\, enzymatic regulation and immune defen
 se mechanism of the single-protein retron system Eco2.
LAST-MODIFIED:20250527T075800Z
LOCATION:MPI Lecture Hall
URL:https://www2.hrz.uni-marburg.de/en/synmikro/grk2937/whats-on/dates-and
 -events/grk2937_speaker-series_patrick-pausch
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