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SUMMARY:Dr. Olivier Duss\, European Molecular Biology Laboratory (EMBL) He
 idelberg: Dynamic RNA biology at the single-molecule level: Watching how i
 nterconnected processes work in real-time
DTSTART;TZID=Europe/Berlin:20250218T090000
DTEND;TZID=Europe/Berlin:20250218T100000
DTSTAMP:20260614T041009Z
UID:7106f907ed984e0a86bbb491ae2c2a9d@www2.hrz.uni-marburg.de
CREATED:20241205T125047Z
DESCRIPTION:https://www2.hrz.uni-marburg.de/en/synmikro/grk2937/whats-on/d
 ates-and-events/grk2937_speaker-series_olivier-duss\nSpeaker Series. A cen
 tral question in biology is how macromolecular machines function cooperati
 vely. \nIn the first part\, I will show our recent work investigating how 
 bacterial transcription and translation cooperate. We have reconstituted a
  complete and active transcription-translation system and developed multi-
 color single-molecule fluorescence microscopy experiments to directly and 
 simultaneously track transcription elongation\, translation elongation and
  the physical and functional coupling between the ribosome and the RNAP in
  real-time (Qureshi & Duss\, Nature\, 2025). A main finding is that the ri
 bosome and the RNAP can communicate with each other by mRNA looping\, prov
 iding an alternative explanation on how the ribosome can efficiently rescu
 e RNAP from frequent pausing without requiring collisions by a closely tra
 iling ribosome. \nIn the second part\, I will discuss our work on understa
 nding how the bacterial rRNA transcription antitermination complex (rrnTAC
 ) coordinates early co-transcriptional rRNA processing. By directly tracki
 ng rrnTAC assembly and co-transcriptional RNase III cleavage in real-time\
 , we show how the presence of the completely assembled rrnTAC facilitates 
 RNase III cleavage by bringing 5’- and 3’-end of the rRNA spatially cl
 ose\, thereby chaperoning the long-range RNA helix which is the substrate 
 for RNase III. This is the first direct experimental evidence of coupling 
 between rRNA transcription and processing in bacterial ribosome assembly m
 ediated by long-range rRNA looping. \nDepending on time\, I may also show 
 some work on our efforts to understand eukaryotic RNA biology\, specifical
 ly how m6A gets introduced into RNA by the Mettl3/14 complex and read in r
 eal-time by the YTHDC1 reader protein.
LAST-MODIFIED:20250127T112132Z
LOCATION:SYNMIKRO Lecture Hall
URL:https://www2.hrz.uni-marburg.de/en/synmikro/grk2937/whats-on/dates-and
 -events/grk2937_speaker-series_olivier-duss
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TZID:Europe/Berlin
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DTSTART:20241027T020000
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